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Edit UltraScan Data

Data Editor Window

All experimental data aquired with the analytical ultracentrifuge require pre-processing to create an edit profile which is necessary for any data to be successfully analyzed with various analysis methods. UltraScan assists you in this process by handling most essential diagnostics and editing steps automatically, but permits you to intervene where necessary. A user can choose to create multiple edit profiles for a particular data set, for example, to explore the effect of various editing steps on the analysis results. For example, a user may wish to exclude either early or late scans to track time dependent changes in the sample. Each profile will receive a unique name based on the time at which the editing process was conducted. For data analysis, any associated edit profile may be selected at run time to provide a custom data selection.

Note: When editing, the plot may be zoomed by selecting the Zoom button on the graph and dragging the mouse over the area desired. This operation may be repeated as needed. Each right mouse click will un-zoom one level. Clicking on the Zoom button again at any zoom level will restore the un-zoomed graph.

When zoomed, the graph may be panned by pressing the center mouse button (usually the scroll wheel) and dragging.

Edit data by following these steps:

  1. Step 1: Load data from the database or a local data directory that contains the UltraScan 3 data files previously converted from the Beckman raw data. Load choices are made in a Load Data Dialog.
  2. Step 2: Select the Cell / Channel / Wavelength triple to be edited.
  3. Step 3: Specify the meniscus of the data by holding down the Control key and using the left mouse button. The meniscus value may be manually adjusted with the keyboard.
  4. Step 4: If the data were collected with the interference detector, specify the left and right edges of the air gap area of the data.
  5. Step 5: Specify the left and right edges of the data to be analyzed. Please note: Do not pick the left data edge too close to the meniscus. During meniscus fitting, the evaluated meniscus positions may reach inside of the data range and violate the boundary conditions of the finite element solution. This will cause the meniscus fit to fail.
  6. Step 6: Specify the location of the scan plateau. This is the radial position where most scans have a stable plateau, but the selected position should not reach into the back-diffusion region. The most appropriate point tends to be close to the right edge of the data range, but not so far to the right that it extends into the region where the concentration of the later scans curves upward at the bottom of the cell due to back-diffusion.
  7. Step 7: Make any other optional adjustments to the data that are necesary and save the edit profile. When saving, a pop-up message is presented asking for an edit ID. The default for this ID is the current date and time in the form of YYMMddhhmm (Year / Month / Day / Hour / Minute), but this default can be supplemented with a suffix of your own choice.

The above process may be reset to any point by pressing the appropriate button at the associated specification entry.

Repeat the above process for each triple (Cell / Channel / Wavelength combination) in the data set.


Multi-WaveLength (MWL) Controls

If the raw data loaded contains multiple wavelengths (more than two), the form of the main window changes to allow an editing sequence oriented towards cell / channel selection and individual wavelength selection ( MultiWavelength Edit ).

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